Pee-wee's Playhouse

Paul Reubens

Pee-wee Herman

Lynne Marie Stewart

Miss Yvonne

Phil Hartman

Captain Carl

Laurence Fishburne

Cowboy Curtis

Gilbert Lewis

The King of Cartoons

Suzanne Kent

Mrs. Renee

S. Epatha Merkerson

Reba

John Paragon

Jambi

Shirley Stoler

Mrs. Steve

Alison Mork

Chairry (voice) / Magic Screen (voice)

Gregory Harrison

Conky 2000 (voice) / Knucklehead (voice)

Natasha Lyonne

Opal

Diane Yang

Cher

William Marshall

The King of Cartoons

Calvert DeForest

Acting

Alisan Porter

Li'l Punkin

Johann Carlo

Dixie

Roland Rodriquez

Tito